منابع مشابه
Efficient transformation of Micromonospora purpurea with pIJ702 plasmid.
The aminoglycoside antibiotic gentamicin is produced by Micromonosporapurpurea strains1 > 2). A gene cloning system for these organisms would be useful both in the study of the organization and regulation of gentamicin biosynthesis genes and, hopefully, for the improvement of the productivity of the strains. Efficient host-vector system and transformation methods have already been developed for...
متن کاملCharacteristics of bacteriophages for Micromonospora purpurea.
Chemical and physical stabilities of bacteriophages øUW 21 and øUW 51 infecting Micromonospora purpurea ATCC 15835 were examined. Both phages were stable over the pH range of 5 to 8 and to heating at temperatures up to 50 degrees C and especially stable in buffer containing magnesium ion. Exposure to 1 M Ca(NO3)2 inactivated both phages, and phage øUW 51 was also susceptible to 1 M CaCl2, 0.1 M...
متن کاملGentamicin formation in Micromonospora purpurea: stimulatory effect of ammonium.
The effect of ammonium on the fermentative production of gentamicin in Micromonospora purpurea has been studied using a chemically defined medium. Ammonium chloride concentrations ranging from 20 to 150 mM resulted in a proportional stimulation of growth and antibiotic formation. The use of other ammonium salts exerted a similar effect. Among the products of ammonium assimilation, glutamate and...
متن کاملEfficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus.
The conditions for optimal formation and regeneration of protoplasts of Streptomyces clavuligerus were established. The optimal temperature for regeneration of protoplasts and for transformation was 26 degrees C in three different regeneration media. The best efficiency of transformation was obtained with 40% polyethylene glycol 1000. The efficiencies of regeneration and transformation increase...
متن کاملA plasmid of the sisomicin producer Micromonospora inyoensis.
pu purea produce gentamicin C and M. in yoensis produces sisomicin1~4). In view of the importance of these organisms it would be useful to attempt to find plasmids in order to establish breeding systems for these organisms. During our preliminary genetic study of _M. in yoensis we isolated a large plasmid. The yield of plasmid DNA was very low and most subisolates did not show plasmids at all. ...
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ژورنال
عنوان ژورنال: The Journal of Antibiotics
سال: 1989
ISSN: 0021-8820,1881-1469
DOI: 10.7164/antibiotics.42.325